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Effect of blood components on gene delivery with polyethylenimine in the cultured endothelial cells

  • At: 2004 FIP Congress in New Orleans (USA)
  • Type: Poster
  • By: SASAKI, Hitoshi (Nagasaki University Hospital, Department of Hospital Pharmacy, Nagasaki, Japan)
  • Co-author(s): Takenaka (Nagasaki University Hospital, NAGASAKI, Japan)
    Nakashima (Nagasaki University Hospital, NAGASAKI, Japan)
    Yoshioka (Nagasaki University Hospital, NAGASAKI, Japan)
    Terazono (Nagasaki University Hospital, NAGASAKI, Japan)
    Ichikawa (Nagasaki University Hospital, NAGASAKI, Japan)
  • Abstract:

    Purpose: The purpose of this study is to investigate the effect of blood components on gene delivery with polyethylenimine (PEI) in a human endothelial cell line EA.hy 926.

    Methods: Plasmid DNA (pDNA) encoding luciferase gene was complexed with PEI at various nitrogen/phosphate (N/P) ratios. For gene transfection experiments, 25000 cells were..

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Last update 4 October 2019

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