Development and validation method for quantitative determination of rifampicin in human plasma and its application in bioequivalence test

  • At: 2017 FIP Congress in Seoul (South Korea)
  • Type: Poster
  • By: LUKITANINGSIH, Endang (Gadjah Mada University, Pharmaceutical Chemistry Department, Yogyakarta, Indonesia)
  • Co-author(s): Endang Lukitaningsih:
    Fathul Jannah: Advanced Pharmaceutical Sciences lab, Gadjah Mada University, Yogyakarta, Indonesia
    Arief Rahman Hakim: Pharmacology and Clinical Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia
    Ratna Dewi Puspita: Gadjah Mada University Hospital, Gadjah Mada University, Yogyakarta, Indonesia
    Zullies Ikawati: Pharmacology and Clinical Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia
  • Abstract:

    Methods

    The chromatographic separation was achieved on a RP-C18 column (LachromHitachi, 250 x 4.6 mm., 5μm), utilizing a mobile phase of phosphate buffer/acetonitrile (55:45, v/v, pH 6.8 ± 0.1) at a flow of 1.5  mL/min. Detection was carried out at 337 nm by using spectrophotometer.

    Results

    The limit of detection and quantification were 0.7 µg/mL

    ..

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