Effect of blood components on gene delivery with polyethylenimine in the cultured endothelial cells

• In: Hospital Pharmacy Section Posters
• At: New Orleans (USA) (2004)
• Type: Poster
• Poster code: HPS-P017
• By: SASAKI, Hitoshi (Nagasaki University Hospital, Department of Hospital Pharmacy, Nagasaki, Japan)
• Co-author(s): Takenaka (Nagasaki University Hospital, NAGASAKI, Japan)
  Nakashima (Nagasaki University Hospital, NAGASAKI, Japan)
  Yoshioka (Nagasaki University Hospital, NAGASAKI, Japan)
  Terazono (Nagasaki University Hospital, NAGASAKI, Japan)
  Ichikawa (Nagasaki University Hospital, NAGASAKI, Japan)
  
• Abstract:

  Purpose: The purpose of this study is to investigate the effect of blood components on gene delivery with polyethylenimine (PEI) in a human endothelial cell line EA.hy 926.
  
  Methods: Plasmid DNA (pDNA) encoding luciferase gene was complexed with PEI at various nitrogen/phosphate (N/P) ratios. For gene transfection experiments, 25000 cells were..

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