Complete knockout of endogenous Mdr1 (Abcb1) in MDCK wild type and MDCK-hMDR1 cells by CRISPR-Cas9

• In: Poster Presentation
• At: Stockholm (Sweden) (2017)
• Type: Poster
• Poster code: P-A-073-Tuesday
• By: KARLGREN, Maria (Uppsala University, Department of Pharmacy, Uppsala, Sweden)
• Co-author(s): Maria Karlgren: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
  Ivailo Simoff: Department of Pharmacy, Uppsala University, Uppsala, Sweden
  Maria Backlund: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
  Anne-Christine Lindström: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
  Christine Wegler: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Cardiovascular Metabolic Diseases DMPK, AstraZeneca R&D, Mölndal, Sweden
  Niklas Handin: Department of Pharmacy, Uppsala University, Uppsala, Sweden
  Patrik Lundquist: Department of Pharmacy, Uppsala University, Uppsala, Sweden
  Markus Keiser: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
  Stefan Oswald: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
  Per Artursson: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
  
• Abstract:

  Backgrounds

  Madin-Darby canine kidney (MDCK) II cells transfected with transport proteins are commonly used for studying drug transport. However, endogenous transporters e.g. canine Mdr1 (Abcb1) complicates the interpretation of transport studies.

  Aims

  The aim of this study was to establish MDCK cell lines lacking endogenous canine Mdr1 (cMdr1)
  ..

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