Inter-laboratory variability in mass spectrometry-based proteomics workflows

• In: Poster Presentation
• At: Stockholm (Sweden) (2017)
• Type: Poster
• Poster code: P-A-108-Monday
• By: WEGLER, Christine (Uppsala University, Department of Pharmacy, Uppsala, Sweden)
• Co-author(s): Christine Wegler: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Cardiovascular Metabolic Diseases DMPK, AstraZeneca, Mölndal, Sweden
  Fabienne Z Gaugaz: Department of Pharmacy, Uppsala University, Uppsala, Sweden
  Tommy B Andersson: Cardiovascular Metabolic Diseases DMPK, AstraZeneca, Mölndal, Sweden
  Jacek R Wisniewski: Department of Proteomics and Signal Transduction, Biochemical Proteomics Group, Max Planck Institute of Biochemistry, Martinsried, Germany
  Stefan Oswald: Center of Drug Absorption and Transport, University Medicine of Greifswald, Greifswald, Germany
  Christian Gröer: Center of Drug Absorption and Transport, University Medicine of Greifswald, Greifswald, Germany
  Diana Busch: Center of Drug Absorption and Transport, University Medicine of Greifswald, Greifswald, Germany
  Frederik Weiss: NMI Natural and Medical Sciences Institut, University of Tuebingen, Tuebingen, Germany
  Helen Hammer: NMI Natural and Medical Sciences Institut, University of Tuebingen, Tuebingen, Germany
  Thomas O Joos: NMI Natural and Medical Sciences Institut, University of Tuebingen, Tuebingen, Germany
  Oliver Poetz: NMI Natural and Medical Sciences Institut, University of Tuebingen, Tuebingen, Germany
  Heleen Wortelboer: TNO, TNO, Zeist, Netherlands
  Evita van de Steeg: TNO, TNO, Zeist, Netherlands
  Brahim Achour: Manchester Pharmacy School, University of Manchester, Manchester, United Kingdom
  Amin Rostami-Hodjegan: Manchester Pharmacy School, University of Manchester, Manchester, United Kingdom
  Per Artursson: Department of Pharmacy, Uppsala University, Uppsala, Sweden
  
• Abstract:

  Backgrounds

  Large variability in protein quantification by a plethora of proteomics workflows has been observed.

  Aims

  The aim was to find causes to variability in membrane protein quantification.

  Methods

  Membrane proteins essential for drug disposition (CYPs, UGTs, SLCs and ABCs) were quantified in ten human liver samples, by six mass
  ..

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