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Construction of human interferon alpha 2b-encoded gene for expression in periplasmic compartment of escherichia coli bl21, characterization and overproduction

  • At: 2014 FIP Congress in PSWC, Melbourne (Australia)
  • Type: Poster
  • By: RACHMAWATI, Heni (Bandung Institute of Technology, Pharmacy, Bandung, Indonesia)
  • Co-author(s): Asmana Ningrum, Ratih (Bandung Institute of Technology, Bandung, Indonesia)
    Jessica, Aditya (Bandung Institute of Technology, Bandung, Indonesia)
    Retnoningrum, Debbie (Bandung Institute of Technology, Bandung, Indonesia)
  • Abstract:

    Interferon alpha2b is a cytokine which is important in human immune response. This research was aimed to develop biosimilar recombinant human interferon alpha2b (rhIFNα2b). To do so, the protein was expressed in periplasm. By this way, rhIFNα2b was transported from cytoplasm to periplasm using enterotoxin II leader peptide. The construction of..

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Last update 4 October 2019

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