Application of titanium dioxide enrichment biotechnology to explore location and biological process of identified mouse sperm phosphoproteins during capacitation

  • At: 2017 FIP Congress in Seoul (South Korea)
  • Type: Poster
  • By: WU, Tzu-Hua (Taipei Medical University, Department of Pharmacy, Taipei, China Taiwan)
  • Co-author(s): Tzu-Hua Wu: Department of Clinical Pharmacy, School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, Province of China
    Jiahn-Haur Liao: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Province of China
    Chang-Shiann Wu: Department of Information Management, National Formosa University, Yunlin, Taiwan, Province of China
    Chien-Sheng Wang: Department of Clinical Pharmacy, School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, Province of China
    Yu-Chun Hung: Department of Clinical Pharmacy, School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, Province of China
    Chia-Te Chou: Department of Clinical Pharmacy, School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, Province of China
    Jhih-Tian Yang: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Province of China
    Shih-Hsiung Wu: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Province of China
  • Abstract:

    Methods

    Titanium dioxide enrichment biotechnology was applied on extracted sperm proteins derived from ICR mice sperm cells. The enrichment of the phosphopeptides was further analyzed by the LTQ Orbitrap Velos instrument operated in data-dependent mode to automatically switch between full scan MS and MS/MS acquisition.

    Results

    207 proteins were..

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