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Complete knockout of endogenous Mdr1 (Abcb1) in MDCK wild type and MDCK-hMDR1 cells by CRISPR-Cas9

  • At: 2017 FIP Congress in Stockholm (Sweden)
  • Type: Poster
  • By: KARLGREN, Maria (Uppsala University, Department of Pharmacy, Uppsala, Sweden)
  • Co-author(s): Maria Karlgren: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
    Ivailo Simoff: Department of Pharmacy, Uppsala University, Uppsala, Sweden
    Maria Backlund: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
    Anne-Christine Lindström: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
    Christine Wegler: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Cardiovascular Metabolic Diseases DMPK, AstraZeneca R&D, Mölndal, Sweden
    Niklas Handin: Department of Pharmacy, Uppsala University, Uppsala, Sweden
    Patrik Lundquist: Department of Pharmacy, Uppsala University, Uppsala, Sweden
    Markus Keiser: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
    Stefan Oswald: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
    Per Artursson: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
  • Abstract:

    Backgrounds

    Madin-Darby canine kidney (MDCK) II cells transfected with transport proteins are commonly used for studying drug transport. However, endogenous transporters e.g. canine Mdr1 (Abcb1) complicates the interpretation of transport studies.

    Aims

    The aim of this study was to establish MDCK cell lines lacking endogenous canine Mdr1 (cMdr1)

    ..

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Last update 4 October 2019

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