Attach or perish: culture morphology and causal factors for variable attachment properties of cryopreserved human hepatocytes
- At: 2017 FIP Congress in Stockholm (Sweden)
- Type: Poster
- By: ÖLANDER, Magnus (Uppsala University, Department of Pharmacy, Uppsala, Sweden)
- Co-author(s): Magnus Ölander: Department of Pharmacy, Uppsala University, Uppsala, Sweden
Jacek Wisniewski: Department of Proteomics and Signal Transduction, Biochemical Proteomics Group, Max Planck Institute of Biochemistry, Martinsried, Germany
Agneta Norén: Department of Surgical Sciences, Uppsala University, Uppsala, Sweden
Per Artursson: Department of Pharmacy, Uppsala University, Uppsala, Sweden
Cryopreserved human hepatocytes are the ‘gold standard’ for hepatic drug disposition studies. Cells from different donors show highly variable attachment properties, and the reasons are largely unclear.
To develop a ‘monolayer score’ for better classification of hepatocyte batches, and to use global proteomics for identifying causes.. The access to the whole abstract and the presentation file is available to FIP members and to congress participants of that specific congress.